How the conversion works
Reconstituted peptide concentration is the labeled mass divided by the bacteriostatic water volume. For a 5 mg vial reconstituted with 2 mL of bacteriostatic water, concentration is 2,500 mcg/mL. A 250 mcg dose at that concentration is 0.10 mL — which on a U-100 syringe is 10 units.
On U-100, U-50, and U-30 insulin syringes, 100 units always equals 1 mL — the smaller barrel size limits the maximum draw, not the unit scale. On U-40 syringes, 40 units equals 1 mL, so the same 0.10 mL draw shows as 4 units.
Limitations
- Estimates assume the labeled vial mass is exact. Manufacturing tolerance is typically 5-10%.
- Dead volume in the syringe and needle (~0.05 mL on a 1 mL insulin syringe) is not accounted for.
- Bacteriostatic water volume displaces a tiny amount of the lyophilized peptide volume; this calculator treats peptide volume as negligible.
- For informational purposes only. Not a substitute for professional medical advice.
Sources
- USP <797> Pharmaceutical Compounding — Sterile Preparations. United States Pharmacopeia.
- Insulin Administration: Standards of Medical Care. American Diabetes Association.